Male Non-obese Diabetic/Severe Combined Immunodeficiency
(NOD/SCID) mice received abdominal irradiation at a single dose of 5-Gy, and then transfused intravenously with cytokines treated Flk-1+MSCs and monitored body diarrhea, weight and survival for 30-day. Colonization and differentiation of transplanted Flk-1+MSCs in the irradiated intestine were analyzed by histological and immunohistochemaical LDK378 in vitro methods. Results: CXCR4 expression in Flk-1+MSCs was up-regulation of by the cytokine cocktail treatment in vitro. The cytokines treated Flk-1+MSCs could significantly extend the life span of the irradiation mice, decrease diarrhea occurrence and improve the small intestinal structural integrity of irradiated mice. Furthermore, XL765 concentration the cytokines treated Flk-1+MSCs were migrated and colonized to the small intestine, and differentiated into fibroblastic-like cells. The immunofluorescence staining showed that the transplanted cells could differentiate into vimentin+/α-SMA+ cells. The mechanism may be that cytokines treatment promoted Flk-1+MSCs home to and engraft to injured sites through up-regulation of CXCR4 expression. In addition, transplanted cells may regulate the epithelial stem/progenitor cells directly or indirectly, and modulate the inflammatory response
through the secretion of trophic factors such as SCF, IL-6 and HGF, which facilitating gastrointestinal repair and gut mucosal barrier function. Conclusion: Our study revealed that administration of cytokines treated Flk-1+MSCs might be a novel therapeutic approach for RIGS. Key Word(s): 1. radiation; 2. MSCs; 3. CXCR4; 4. transplantation; Presenting Author: XUDONG TANG Additional Authors: LIYA ZHOU, ZHENHUA LI, SHUTIAN ZHANG, BIN LV, JUNXIANG LI, BAOSHUANG LI, HUIZHEN Unoprostone LI Corresponding Author: XUDONG TANG Affiliations: Xiyuan Hospital affiliated to China Academy of Traditional Chinese Medicine; Peking University Third Hospital; Beijing Friendship Hospital affiliated to Capital Medical University; Zhejiang Hospital of Traditional Chinese Medicine; Dongfang Hospital affiliated to Beijing University of Traditional Chinese Medicine; The No 2.Hospital affiliated
to Tianjin University of Traditional Chinese Medicine Objective: To evaluate the effect and safety of Chinese herbal medicine based on syndrome differentiation and Moluodan on the gastric epithelial dysplasia in patients with chronic atrophic gastritis. Methods: This is a multi-centered, randomized, clinical controlled trial. Patients in western hospitals and traditional Chinese medicine (TCM) hospitals were given Moluodan or herbal medicine, compared with folic acid respectively. Three hundred and eighty-three subjects with atrophic gastritis accompanied with dysplasia were consecutively enrolled from Beijing and Hangzhou in China, 125 patients in Chinese herbal medicine group, 130 in Moluodan group, and 128 in folic acid group.