Results The 90 patients had received a median 156 weeks of NUCs treatments before EOT.

Seventy patients (77.8%) achieved the recommended APASL treatment endpoint, including 15 (53.6%) HBeAg-positive and 55 (88.7%) HBeAg-negative patients. During the median follow-up period of 36.6 weeks (range 3 to 102 weeks), VR and CR developed in 71.1% and 37.8% of patients, respectively. In HBeAg-positive patients, the median time to VR and CR were 24.1 and 66.4 weeks, respectively. There was no significant predictor Dabrafenib mouse of VR, while achieving APASL treatment endpoint was the only predictor of CR (HR = 0.127, p = 0.014). In the 15 HBeAg-positive patients who achieved APASL treatment endpoint, 8 (53.3%) and 3 (20%) patients still developed Wnt inhibition VR and CR, respectively. In HBeAg-negative patients, the median time to VR and CR were 31.9 and 74.7 weeks, respectively. Neither achieving APASL treatment endpoint nor low qHBsAg level at EOT were not associated with VR and CR. In the 16 HBeAg-negative patients with HBsAg <200 IU/mL at EOT, 11 (68.8%) and 3 (18.8%) patients still developed VR and CR, respectively. Conclusions The risk of VR is high after cessation of NUCs treatment even achieving APASL treatment endpoint in both HBeAg-positive and –negative CHB patients.

Low HBsAg level at EOT could not confer relapse-free status. HBV viral load should be closely monitored for all patients after cessation of NUCs. Disclosures: The following people have nothing to disclose: Yi-Hsiang Huang, I-Cheng

Lee, Cheuk-Kay Sun, Chien-Wei Su, Yuan-Jen Wang, Han-Chieh Lin Background Chronic hepatitis B (CHB) remains a serious public health burden, especially in Southeast Asia. The approved antiviral drugs for CHB treatment include nucleotide analogs and interferons (IFNs), both of which are effective in selected patients and limited by resistance or considerable side-effect. Herbs have increasingly drawn attention as potential sources of antiviral drugs. Among them, Dandelion belongs to the Compositae family and one of its components is traxasterol. It has been reported that dandelion extracts possessed anti-influenza virus properties and traxasterol inhibited Epstein-Barr virus early antigen. The aim of present study was to investigate the inhibitory effect and underlying mechanism of dandelion extract and traxasterol on HBV replication medchemexpress in vitro. Methods Dandelion or traxasterol was added to human hepatoblastoma cell line which are stably transfected with HBV clone-HepG2.2.15, with lamivudine as a positive control. After culture, supernatants were collected to measure HBV DNA, pregenomic (pg) RNAs, HBsAg and HBeAg by reverse transcription PCR (qRT-PCR) or commercial enzyme-linked immunoassay. Intracellular HBsAg and HBcAg expression were determined by immunofluorescence and western blotting. And the level of polypyrimidine tract binding protein (PTB) expression was determined by western blotting.