J Bacteriol 2009, 191:3657–3664.PI3K inhibitor PubMedCrossRef 77. Barnéoud-Arnoulet A, Barreteau H, Touzé T, Mengin-Lecreulx D, Lloubès R, Duché D: Toxicity of the colicin M catalytic domain exported to the periplasm is FkpA independent. J Bacteriol 2010, 192:5212–5219.PubMedCrossRef 78. Barreteau H, Bouhss buy CA4P A, Gérard F, Duché D, Boussaid B, Blanot D, Lloubès R, Mengin-Lecreulx D, Touzé T: Deciphering the catalytic domain of colicin M, a peptidoglycan lipid II-degrading enzyme. J Biol Chem 2010, 285:12378–12389.PubMedCrossRef 79. Breukink E, de Kruijff B: Lipid II as a target for antibiotics.

Nat Rev Drug Discov 2006, 5:321–323.PubMedCrossRef 80. Budič M, Rijavec M, Petkovšek Ž, Žgur-Bertok D: Escherichia coli bacteriocins: antimicrobial efficacy and prevalence among isolates from patients with bacteraemia. PLoS One 2011, 6:e28769.PubMedCrossRef 81. Anderluh G, Gökçe I, Lakey JH: Expression of proteins using the third domain of the Escherichia coli periplasmic-protein TolA as a fusion partner. Protein Expr Purif 2003, 28:173–181.PubMedCrossRef 82. Benjamini selleck screening library Y, Hochberg Y: Controlling the false discovery rate: a practical and powerful approach to multiple testing. J Roy Stat Soc B Met 1995, 57:289–300. 83. Rozen S, Skaletsky H: Primer3 on the WWW for general users and for biologist programmers. Methods Mol Biol 2000,

132:365–386.PubMed 84. Livak KJ, Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2 −ΔΔ C T method. Methods 2001, 25:402–408.PubMedCrossRef 85. Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy click here N, De Paepe A, Speleman F: Accurate normalization of real-time quantitative RT-PCR data by

geometric averaging of multiple internal control genes. Genome Biol 2002, 3:RESEARCH0034.PubMedCrossRef 86. Obadia B, Lacour S, Doublet P, Baubichon-Cortay H, Cozzone AJ, Grangeasse C: Influence of tyrosine-kinase Wzc activity on colanic acid production in Escherichia coli K12 Cells. J Mol Biol 2007, 367:42–53.PubMedCrossRef 87. Rijavec M, Müller-Premru M, Zakotnik B, Žgur-Bertok D: Virulence factors and biofilm production among Escherichia coli strains causing bacteraemia of urinary tract origin. J Medical Microbiol 2008, 57:1329–1334.CrossRef 88. Miller JH: Experiments in molecular genetics: Assay of β-galactosidase. Cold Spring Harbor: CSH Laboratory Press; 1972:352–355. Competing interests The authors declare that they have no competing interests. Authors’ contributions Conceived and designed the experiments: DŽB. Performed the experiments: SK. Analyzed the data: SK, DŽB. Contributed reagents/materials/analysis tools: SK, DŽB. Wrote the paper: SK, DŽB. Both authors read and approved the final manuscript.”
“Background Streptococcus agalactiae or group B streptococcus (GBS) is the major cause of invasive neonatal infections in industrialized countries [1, 2].