The growth of cells were significantly inhibited by SWNHs at each time point PF-6463922 cell line in a dose-dependent manner (P < 0.001), especially in cells pre-treated with LPS (B). Cell viability was evaluated by CCK-8 assay. The result showed that the proliferation of N9 cells pre-treated with LPS (D) was much more significantly than N9 cells (C). The proliferation of N9 cells treated with SWNHs was significantly inhibited at each time point in a time and dose-dependent manner (C and D). The effect induced by SWNHs on N9 cells pre-treated with LPS (D) was far more than that cells pre-treated without LPS (B). All data are represented as mean ± SEM. Cell viability was

evaluated by CCK-8 assay. The result showed that the proliferation of N9 cells pre-treated with LPS (Figure 2D) was much more significant than that in N9 cells (Figure 2C). The proliferation of N9 cells treated with SWNHs was significantly inhibited at each time point in a time- and dose-dependent manner (Figure 2C,D). The effect induced by SWNHs on N9 cells pre-treated with LPS (Figure 2D) was far more significant than that cells pre-treated without LPS (Figure 2B).

SWNHs SNX-5422 in vivo affected cell cycle of N9 cells, especially in pre-treated with LPS The cell cycle of N9 cells was affected by SWNHs in a dose-dependent manner, especially in cells pre-treated with LPS (Figure 3B). Followed with the increasing Cediranib (AZD2171) RAD001 ic50 concentrations of SWNHs, the ratio of the G1 phase increased and S phase decreased significantly in N9 cells pre-treated with LPS (P < 0.01). The ratio of G2 phase decreased in N9 cells and it decreased until SWNHs30 and increased abruptly at the concentration of SWNHs40

in N9 cells pre-treated with LPS (P > 0.05). The effect induced by SWNHs on N9 cells pre-treated with LPS was more significant than on N9 cells (Figure 3A). Figure 3 SWNHs affected cell cycle of N9 cells, especially in pre-treated with LPS. Cell cycle of N9 cells was affected by SWNHs in a dose-dependent manner, especially in cells pre-treated with LPS (B). Followed with the increasing concentrations of SWNHs, the ratio of the G1 phase increased and S phase decreased significantly in N9 cells pre-treated with LPS (P < 0.01), the ratio of G2 phase decreased in N9 cells and it decreased until SWNHs30 and increased abruptly at the concentration of SWNHs40 in N9 cells pre-treated with LPS (P > 0.05). The effect induced by SWNHs on N9 cells pre-treated with LPS was more significant than on N9 cells. All data are represented as mean ± SEM. SWNHs promoted cell apoptosis of N9 cells, especially in pre-treated with LPS After the cells had been cultured onto SWNHs-coated dishes for 48 h, the effect of SWNHs on cell apoptosis distribution was determined by flow cytometry.