Electrophysiological mapping revealed the orderly digit topography in area 3b and area 1 (Figure 5A). Consistent with our previous studies (Friedman et al., 2004, 2008), optical imaging of cortical activation in response to stimulation of single digit tips revealed two activation spots, one in area 3b and one in area 1 (response to D2 stimulation shown in Figure 5B). A focal injection of BDA confined to the single digit-tip representation (<500 μm in diameter)

(Figure 5C) was made in the D2 tip location, and the resulting cellular label was reconstructed (Figures 5D and BMS354825 6). The injection resulted in heavy labeling of cells (orange and yellow) near the injection site in area 3b, as well as patchy label (green and blue) distant from the injection site in the hand area in area 3b (Figure 5D; see also Figure 6). These included adjacent digit locations within area 3b in distal D1, D3, and D4. Heavy label was also observed in area 1, predominantly in the D2/D3 region with heavy focus in the tip representation zone (Figure 5D). Consistent with reciprocal connectivity patterns in somatosensory cortex, BDA-labeled axonal terminal patches (Figure 6) were also observed to share a similar pattern of connectivity (Négyessy et al.,

2013). Thus, the labeling in this case suggests topographically widespread inputs from other digit locations within area 3b, and relatively mediolaterally restricted inputs from area 1, from largely topographically matched locations. This differential intra- versus interareal Nintedanib solubility dmso pattern of labeling to was also seen in two other cases (Figures 5E and 5F; see also Figure 6). Thus, anatomical connections were characterized by two primary axes of information flow (broad intra-areal [Figures 5D–5F, curved red arrows] and comparatively focused interareal connectivity [Figures 5D–5F, straight red arrows]). This pattern was consistent

with the strong digit-matched resting-state connectivity between area 3b and area 1, the weaker but distinct connectivity between different digits within area 3b, and the even weaker connectivity between nonmatching digits between area 3b and area 1 (Figure 3E). These patterns of connectivity within area 3b and between areas 3b and 1 also were supported by electrophysiological recordings of steady-state neuron-neuron interactions in four other squirrel monkeys. After optical imaging and electrophysiology mapping (Figures 7A and 7B), on separate electrodes, single units were isolated in the digit-tip representations (D2, D3, and D4 tips) of area 3b and area 1. Area 3b-area 1 (A3b-A1) pairs were either same-digit or adjacent-digit pairs; area 3b-area 3b (A3b-A3b) pairs were all adjacent-digit pairs.