Method detection limits (MDLs) for the emissions tests ranged from 0.03 to 0.5ng TDI g(1) foam (0.0020.04ng TDI cm(2) of foam surface), whereas those for the migration tests were 0.73ng TDI g(1) foam (0.16ng TDI cm(2) of foam surface). Of the three emission test methods used, the FLEC had the lowest relative MDLs (by a factor of 310) by virtue of its high chamber loading

factor. In addition, the FLEC cell offers well-established conformity with emission testing standard methods.”
“The purpose of this systematic literature review was to describe the empirical findings on health literacy in adults 65 and older. A systematic search for research studies AR-13324 from 1999 to early 2010 was conducted in MEDLINE and CINAHL databases. Articles were analyzed using the following foci: skills and health knowledge, functional health literacy, and health behaviors and health outcomes. Findings indicated that advancing age resulted in a significant increase in prevalence of inadequate health literacy. Further research is needed to develop evidence-based, culturally congruent interventions to improve health literacy among older adults.”
“A typical plasmid replicon of Escherichia

coli, such as ori gamma of R6K, contains tandem iterons (iterated initiator protein binding sites), an AT-rich region that melts upon initiator-iteron interaction, two binding sites for the bacterial initiator protein DnaA, and a binding HSP inhibitor site for the DNA-bending VX-689 protein IHF. R6K also contains two structurally atypical origins called gamma and beta that are located on either side of gamma and contain a single and a half-iteron, respectively. Individually, these sites do not bind to initiator protein pi but access it by DNA looping-mediated interaction with the seven pi-bound gamma iterons. The pi protein exists in 2 interconvertible forms: inert dimers and active monomers. Initiator

dimers generally function as negative regulators of replication by promoting iteron pairing (“handcuffing”) between pairs of replicons that turn off both origins. Contrary to this existing paradigm, here we show that both the dimeric and the monomeric pi are necessary for ori alpha-driven plasmid maintenance. Furthermore, efficient looping interaction between alpha and gamma or between 2 gamma iterons in vitro also required both forms of pi. Why does alpha-gamma iteron pairing promote alpha activation rather than repression? We show that a weak, transitory alpha-gamma interaction at the iteron pairs was essential for alpha-driven plasmid maintenance. Swapping the alpha iteron with one of gamma without changing the original sequence context that caused enhanced looping in vitro caused a significant inhibition of alpha-mediated plasmid maintenance. Therefore, the affinity of alpha iteron for pi-bound gamma and not the sequence context determined whether the origin was activated or repressed.