CD25 of SKOV3/tk-MCP-1 was significantly higher than that of SKOV3/tk (P < 0.05). CD44v6 of SKOV3/tk (6.66 ± 2.01%) and SKOV3/tk-MCP-1 (6.51 ± 1.03%) was FAK inhibitor significant lower than that of control group (40.74 ± 3.58%) (P < 0.01). Figure 3 CD 25 of SKOV 3 /tk (20.00 ± 2.04%) and SKOV 3 /tk-MCP-1 (38.82 ± 2.48%) was obviously higher than SKOV 3 /neo (8.73 ± 1.65%) ( P < 0.05). CD25 of SKOV3/tk-MCP-1 was significantly
higher than that of SKOV3/tk (P < 0.05). CD44v6 of SKOV3/tk (6.66 ± 2.01%) and SKOV3/tk-MCP-1 (6.51 ± 1.03%) was significantly lower than that of control group (40.74 ± 3.58%) (P < 0.01) Antitumor effects of recombinant gene in vivo Forty SCIDs (IgG < 5 μg/ml) were injected PBMC intraperitoneally. Three weeks later immune reconstruction was successfully established in SCID mouse (human IgG > 5 μg/ml). The ratio of successful tumor MK-8931 mw transplantation
was 100%. The tumor was widespread in peritoneal cavity. The reduction of abdominal bulge and the improvement of spirit and appetite of tk-MCP-1group were greater than tk or MCP-1, and the condition of control group had no amelioration. The survival period of tk-MCP-1 group was significantly longer than tk or MCP-1 group, followed by the control group (35 ± 2.94 d, 25 ± 2.16 d, 26 ± 2.58 d and 15 ± 3.16 d, P < 0.05). There was no significant difference between tk and MCP-1 groups (P > 0.05) (Figure 4-F). The ovarian tumors of the tk-MCP-1 group shrank significantly, MLN2238 in vitro followed by the tk or MCP-1 group. However, the tumor of the control very group was still widespread in peritoneal cavity and cavitas pelvis There was no significant difference between tk and MCP-1 groups (P > 0.05) (Figure 4A–E). As shown in Figure 5 and Table 2, flow cytometry examination revealed that the number of macrophages infiltrated the tumor
tissues in the control group, tk or MCP-1 group and tk-MCP-1 group increased in order (P < 0.05), so did TNF-α protein level from the activated microphages. There was no significant difference between tk and MCP-1 groups (P > 0.05). Figure 4 A–E. The ovarian tumors of the tk-MCP-1 group shrank significantly, followed by the tk or MCP-1 group. However, the tumor of the control group was still widespread in peritoneal cavity and cavitas pelvis. F. Kaplan-Meier survival analysis of mice intraperitoneally transplanted with diverse tumor cells. a. SKOV3/tk-MCP-1 b. SKOV3/MCP-1 c. SKOV3/tk d. SKOV3/neo. Figure 5 Flow cytometry examination revealed that the number of macrophages (A) infiltrated the tumor tissues in the control group, tk or MCP-1 group and tk-MCP-1 group increased in order ( P <0.05), so did TNF-α protein level from the activated microphages. There was no significant difference between tk and MCP-1 groups (P > 0.05) (B). a. SKOV3/neo b. SKOV3/tk c. SKOV3/MCP-1 d. SKOV3/tk-MCP-1.