, 2005). Whilst reductions in bacterial

susceptibility have been observed following repeated exposure (Perron et al., 2003), HDPs are reportedly less likely to induce bacterial resistance, in comparison with conventional antibiotics (Steinberg et al., 1993; Ge et al., 1999; Mosca et al., 2000). Human salivary HDPs comprise various short-chain peptides that are commonly associated with mucosal surfaces and which exhibit broad-spectrum antimicrobial activity. They have been classified into three subcategories: defensins, histatins and cathelicidin LL37 (Boman, 2000); defensins and cathelicidins constitute < 1% of total salivary proteins, whilst histatins constitute c. 5% (van Nieuw Amerongen et al., 2009). find protocol Oral HDPs are derived from various sources (Fig. 1) including neutrophils, which produce human neutrophil proteins (HNPs) (Selsted et al., 2009, 1992); gingival epithelia, which produce β defensins (Krisanaprakornkit et al., 1998); and salivary glands that secrete Everolimus in vivo histatins (Imamura et al., 2009) (Fig. 1). HDP production levels may vary in a stimulus-dependent manner (reviewed by Dale & Fredericks, 2005; Gorr & Abdolhosseini, 2011) and their

biological functions include chemotaxis, where HNPs 1 and 2 for example attract monocytes (Territo et al., 2000); stimulation of epithelial cell turnover during wound healing; neutralization of bacterial lipopolysaccharides; antiviral activity, and direct antibacterial effects (Dimond et al., 2009). Antibacterial activity occurs by interaction with the cell envelope, causing progressive leakage of cell contents (Zasloff, 2002).

Previous investigations into the antimicrobial activity of human oral HDPs suggest that they exhibit varying potency against oral bacteria when pure cultures are exposed in endpoint susceptibility tests (Hancock & Devine, 2004; Dimond et al., 2009). The role of HDPs in influencing the microbiological composition Carnitine dehydrogenase of the oral microbiota has been suggested by investigations of human subjects. For example, a single nucleotide polymorphism in Type I diabetics which reduces the efficacy of β defensin 1 has been associated with increased carriage of the pathogenic yeasts Candida glabrata and Candida tropicalis (Jurevic et al., 2003), whilst salivary proteomics of diabetic children has revealed a lack of histatins, which has been correlated with an increased periodontitis incidence (Cabras et al., 2010). In Chediak–Higashi syndrome, where individuals lack neutrophil azurophilic granules (a major source of HDPs), elevated susceptibility to bacterial and fungal infections has been reported (Ganz et al., 1988). Finally, levels of LL37 increase in response to the progression of periodontitis, and this HDP may therefore act as an inducible protective factor (Turkoglu et al., 1989).