A total of 46 responses were diagnostic between at least some of the species and are listed in Table 3. Results for identification of P. minutispora and Petriellopsis africana from which only single strains were analysed are only included in the Table if they were remarkable and therefore usable as specific identification markers. Scedosporium prolificans was clearly CP-673451 nmr distinguishable from remaining species by nine compounds (l-valine, p-aminohippuric acid, adonitol, dulcitol, sedoheptulose, β-d-glucosamine, glycine-tryptophan-βNA and d-alanine-para-naphthylamide

(pNA), bolded values in Table 3). The single P. minutispora isolate was the only strain positive for γ-hydroxybutyrate. l-Asparagine and l-glutamine distinguished P. minutispora and Petriellopsis africana. Additional species-specific reactions were acid production Dinaciclib chemical structure from sucrose for the differentiation of S. aurantiacum (–) from all other species of the P. boydii complex (+), assimilation of glycine-glycine-βNA, sucrose-phenylanaline-glycine-leucine-βNA, and praline-pNA for differentiation of S. aurantiacum (+) and S. dehoogii (–) as well as assimilation of p-nitrophenyl-β-d-maltoside (pH 7.5) and p-nitrophenyl-β-d-glucopyranoside (pH 5.5) for separation of P. boydii (–) from S. aurantiacum (+). Pseudallescheria apiosperma could be distinguished from the P. boydii complex only by a combination of characters obtained with p-nitrophenyl-α-l-rhamnopyranoside

(pH 7.5), p-nitrophenyl-β-d-maltoside (pH 7.5) and p-nitrophenyl-β-d-glucopyranoside (pH 5.5). Intraspecific variability was present in all species for which more than one strain was analysed. In Table 4, the numbers of species-specific positive,

negative and variable results of each species from which more than one isolate was available for study are listed. The lowest degree of variation regarding all reactions was found in S. aurantiacum (22.5%) and in S. prolificans (27.2%). Variabilities of P. boydii (53.5%), P. Miconazole apiosperma (49.2%) and S. dehoogii (48.4%) were in the same range. Especially in P. apiospermua, large differences were found in the variability of the different Taxa Profile microtitre platforms: 61.3% in Profile A (amino derivates), 25.6% in Profile C (carbohydrates) and 59.8% in Profile E (aminopeptidases, glucosidases, phosphatases) respectively. The environmental strain CBS 467.76 of S. prolificans differed from clinical isolates of this species by positive results for catechol, 3-aminobenzamide, gum xanthan, pectin and negative results for protocatechuate, asparagine-βNA, hypoxanthine-βNA-HCl, glutaminic acid-glutaminic acid-βNA, glutaminic acid-histidine-βNA and histidine-leucine-histidine-βNA. Both algorithms for cluster analysis (SSM and SJ) generated seven robust clusters, with S. prolificans in a remote position. The dendrogram constructed from SSM analysis is presented in Fig. 1.