All of the studies had at least two study arms in which one group Hydroxychloroquine datasheet of patients received PI PCs, while the other received standard PCs. The participants in these trials were predominantly hemato-oncology patients who were receiving prophylactic transfusion protocols in a setting of post-chemotherapy thrombocytopenia; the study periods ranged from 28 to 56 days. One of the principal stakes of these studies rested on the definition of the primary outcome. The more

common outcome used was the change in CCI. The CCI indicates the increase in platelet count after transfusion, corrected for the number of platelets transfused and the body surface area of the recipient. This formula was originally used to define refractory state to platelet transfusion; as such, it is not an intrinsic quality parameter for platelet products [80]. CCI has the advantage of easy measurement and allows for quantitative comparisons. However, it has not been established that this measure is of clinical relevance. For example, in the PLADO study, although the CCIs were different in three groups of patients who received 1.1 × 1011, 2.2 × 1011, and 4.4 × 1011 platelets/m2, respectively, the clinical outcomes were similar [81].

The SPRINT trial was the only trial to use the bleeding score, as defined by the World Health Organization (WHO), as the primary outcome measure [77]. Other clinical criteria, such as the find more HA-1077 cell line number of PC and RBC transfusions and the time interval between two transfusions, have been used as secondary outcomes, together with the TR rate, the appearance of neoantigens, and the risk of platelet alloimmunization. In addition to how clinically relevant outcomes are defined, numerous other biases may arise in association with the methods used in the aforementioned studies. Possible pitfalls were described by Cook and Heddle in their review of the methodology

of clinical trials with patients transfused with PI-treated PCs [82]. The very characteristics of the PCs varied among the studies, making it difficult to compare the study results: platelets were obtained through apheresis or prepared from buffy coats (in Europe) or platelet-rich plasma (in the USA), the number of platelets per bag and the composition of the additive solution differed, the shelf life was variable, and the presence or absence of γ-irradiation and the transfusion threshold was substantially different from one study to another. Part of the variability may also be patient linked, although the exclusion criteria generally contained risk factors for platelet refractoriness, such as splenomegaly, HLA or HPA alloimmunization, and the presence of disseminated intravascular coagulopathy.