Alonso MA, Millan J: The role of lipid rafts in signalling and membrane trafficking in T lymphocytes. Journal of cell science 2001, 114 (Pt 22) : 3957–3965.PubMed 26. Schwartz DR, Kardia SL, Shedden KA, Kuick R, Michailidis G, Taylor JM, Misek DE, Wu R, Zhai Y, Darrah DM, et al.: Gene expression in ovarian cancer reflects both morphology and biological behavior, distinguishing clear cell from other poor-prognosis ovarian carcinomas. Wnt tumor Cancer research 2002, 62 (16) : 4722–4729.PubMed Competing interests The authors declare that they have no competing interests. Authors’ buy Pitavastatin contributions Wei Yan, Qing Li, Feng

Zhu and Ruian Wang designed and supervised the experiments. Wei Yan contributed to pathologic morphological diagnosis. Qinlong Li, Kainan Li and Wenyong Wang carried out plasmid construction and cell transfection. Yaqing Zhang, Weihuang Wang and Jihong Cui performed immunohistochemistry. Yaqing selleck chemicals Zhang, Qinlong Li and Wei Yan performed the statistic analysis and drafted the manuscript. All authors have read and approved the final version of the manuscript.”
“Background Lung cancer is the number one cause of cancer mortality in both males and females worldwide [1]. Despite multidisciplinary treatment, lung cancer is still a highly lethal disease due to late detection and resistance to chemotherapy. The identification of new therapeutic agents that exert

synergistic effects in combination with traditional cytotoxic agents is an alternative strategy for the systemic treatment of lung cancer. Recent evidence

Non-specific serine/threonine protein kinase indicates that arsenic trioxide (As2O3) may induce clinical remission in patients with acute promyelocytic leukemia (APL), and several investigations show that As2O3 induced programmed cell death in APL cell lines [2–5]. DDP, a platinum-containing anticancer drug, is one of the most commonly used cytotoxic agents for the treatment of lung cancer. Due to the poor therapeutic effects of current cytotoxic-agents on lung cancer, the ability of As2O3 to induce apoptosis in non-small cell lung cancer cells was explored in the present study, and the synergistic effects of As2O3 with DDP on A549 and H460 lung cancer cells were analyzed. Methods Cell culture and reagents Human lung cancer A549 and H460 cell lines were obtained from the ATCC and maintained in RPMI 1640 medium with 10% fetal bovine serum and 1% penicillin. As2O3 was purchased from Yida Pharmaceutical Co.(GMP, Ha’erbin, PR. China) and DDP was from Bristol-Myers Squibb Co.(Shanghai, PR. China). MTT assay Briefly, cells were seeded at a density of 2,000 to 5,000 cells/well in 96-well plates and incubated overnight. After treatment with As2O3, DDP, or their combination (described below), 3-(4, 5-methylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) was added (50 μL/well) for 4 hours. Solubilization of the converted purple formazan dye was accomplished by placing cells in 100 μL of 0.01 N HCl/10% SDS and incubating them overnight at 37°C.