PubMedCrossRef 17. Hayashi T, Ueda S, Tsuruta H, Kuwahara H, Osawa R: Complexing of green tea catechins with food constituents and degradation of the complexes by Lactobacillus plantarum . Bioscience of Microbiota, Food and Health 2012, 31:27–36.CrossRef 18. Schrag JD, Li YG, Wu S, Cygler M: Ser-His-Glu triad forms the catalytic site

of the lipase from Geotrichum candidum . Nature 1991, 351:761–764.PubMedCrossRef 19. Ren B, Wu M, Wang Q, Peng X, Wen H, McKinstry WJ, Chen Q: Crystal IACS-10759 cost Structure of Tannase from Lactobacillus plantarum . J Mol Biol 2013, 425:2731–2751.CrossRef 20. Banerjee A, Jana A, Pati BR, Mondal KC, Das Mohapatra PK: Characterization of tannase protein sequences of bacteria and fungi: an in silico study. Protein J 2012, 31:306–327.PubMedCrossRef

21. Rodríguez H, de las Rivas B, Gómez-Cordovés C, Muñoz R: Characterization of tannase activity in cell-free extracts of Lactobacillus plantarum CECT 748 T. Int J Food Microbiol 2008, 121:92–98.PubMedCrossRef 22. Watanabe K, Masuda T, Ohashi H, Mihara H, Suzuki Y: Multiple proline substitutions PS-341 cumulatively thermostabilize Bacillus cereus ATCC7064 oligo-1,6-glucosidase. Irrefragable proof supporting the proline rule. Eur J Biochem 1994, 226:277–283.PubMedCrossRef 23. Sawatari Y, Yokota A: Diversity and mechanisms of alkali tolerance in lactobacilli. Appl Environ Microbiol 2007, 73:3909–3915.PubMedCentralPubMedCrossRef 24. Sánchez AH, Rejano L, Montaño A, de Castro A: Utilization at high pH of starter cultures of lactobacilli for Spanish-style green olive fermentation. Int J Food Microbiol 2001, 67:115–122.PubMedCrossRef 25. Yao J, Fan XJ,

Lu Y, Liu YH: Isolation and characterization of a novel tannase from a metagenomic library. J Agric Food Chem 2011, 59:3812–3818.PubMedCrossRef 26. Rajakumar GS, Nandy SC: Isolation, purification, and some properties of Penicillium chrysogenum tannase. Appl Environ Microbiol 1983, 46:525–527.PubMedCentralPubMed 27. Smith AH, Zoetendal E, Mackie RI: Bacterial mechanisms to overcome inhibitory effects of dietary tannins. Microb Ecol 2005, TCL 50:197–205.PubMedCrossRef 28. Bhatia Y, Mishra S, Bisaria VS: Microbial β-Glucosidases: cloning, properties, and applications. Crit Rev Biotechnol 2002, 22:375–407.PubMedCrossRef Competing interests The authors declare that they have no competing interests. Authors’ contributions SU carried out the molecular genetic studies and this website enzymatic analysis, participated in the sequence alignment, purification the recombinant enzymes, and kinetic analysis. RN performed the data analysis, participated in the design of the study, and drafted the manuscript. KY helped to draft the manuscript. RO conceived of the study, and participated in its design and helped to draft the manuscript. All authors read and approved the final manuscript.”
“Background Citrus Huanglongbing (HLB), literally from the Chinese “Yellow Shoot Disease”, is one of the most devastating diseases that threaten citrus production worldwide [1].