Figure 2 Positive immunostaining of positive controls in breast cancer tissues (original magnification ×200). Association
of NUCB2 protein expression with the clinicopathological check details variables of PCa We investigated the association between NUCB2 protein expression status and commonly used clinicopathological variables in PCa. The associations between NUCB2 protein expression and clinicopathological variables are shown in Table 2. High expression of NUCB2 protein was significantly associated with seminal vesicle invasion (P = 0.016), the higher level of preoperative PSA (P = 0.006), positive lymph node metastasis AZD5582 nmr (P = 0.022), the positive angiolymphatic invasion (P = 0.042), BCR, and the higher Gleason score (P = 0.017). However, the NUCB2 protein expression was not associated with age, pathological stage, and surgical margin status. Table 2 Clinicopathologic variables and NUCB2 protein expression in 180 PCa patients Variable Group NUCB2 protein expression P value n High Low Age 0.897 <70 97 54 (55.7%) 43 (44.3%) ≥70 83 47 (56.6%) 36 (43.4%) Lymph node metastasis Selleck PI3K Inhibitor Library 0.022 Positive 17 14 (82.4%) 3 (17.6%) Negtive 163 87 (53.4%) 76 (46.6%) Surgical margin status 0.521 Positive 14 9 (64.3%) 5 (35.7%) Negtive 166 92 (55.4%) 74 (44.6%) Seminal vesicle invasion 0.016 Positive 35 26 (74.3%) 9 (25.7%) Negtive 145 75 (51.8%) 70 (48.3%) PCa stage 0.114 T1 103
63 (61.2%) 40 (38.8%) T2/T3 77 38 (49.4%) 39 (50.6%) Preoperative PSA 0.006 <4 5 1 (20%) 4 (80%) 4-10 64 28 (43.8%) 36 (56.2%) >10 111 72 (64.9%) 39 (35.1%) Gleason score 0.017 <7 99 47 (47.5%) 52 (52.5%) 7 34 20 (58.8%) 14 (41.2%)
>7 47 34 (72.3%) 13 (27.7%) Angiolymphatic invasion 0.042 Positive 35 25 (71.4%) 10 (28.6%) Negtive 145 76 (52.4%) 69 (47.6%) Biochemical recurrence 0.003 Absence 128 63 (49.2%) 65 (50.8%) Presence 52 38 (73.1%) 14 (26.9%) Correlation of NUCB2 protein expression with BCR-free survival To investigate the prognostic value of NUCB2 for PCa, we assessed the association between the NUCB2 protein expression and the BCR-free survival duration using a Kaplan–Meier analysis BCKDHB with a log-rank test. The log-rank test showed that the BCR-free survival time of patients with PCa was significantly different between the groups with high NUCB2 protein expression and low NUCB2 protein expression. In patients with PCa, the high NUCB2 protein expression group had a shorter survival duration compared to the low NUCB2 protein expression group. Univariate analysis with Cox proportional hazards model identified 3 prognostic factors: seminal vesicle invasion (P = 0.005), Gleason score (P < 0.001), and NUCB2 protein expression (P < 0.001). The other clinicopathological features, such as age, preoperative PSA, angiolymphatic invasion, lymph node metastasis, surgical margin status, and pathological stage were not statistically significant prognosis factors.