In the present study we localised G(s)-protein-coupled DP1 and G(i)-protein-coupled DP2 receptors in DRG neurons, and we assessed the effect of PGD(2) on TTX-R Na+ currents in patch-clamp recordings from small-to medium-sized

cultured DRG neurons from adult rats. DP1 and DP2 receptor-like immunoreactivity was localised in the vast majority of DRG neurons. In all neurons, PGD(2) shifted conductance to more hyperpolarised potentials, depending on an action at Na(v)1.9 channels. In about one third of the neurons, PGD(2) additionally influenced Na(v)1.8 channels by facilitating conductance and by increasing maximal current amplitudes. Selective DP1 receptor activation increased the amplitude of TTX-R Na+ currents of most neurons, but this effect was counteracted by DP2 receptor activation, find more which by itself had no effect. In the current-clamp mode, PGD(2) lowered the threshold AZD1208 JAK/STAT inhibitor for elicitation of an action potential and increased the number of action potentials per stimulus, an effect mainly depending on DP1 receptor activation. Thus, the net effect of PGD(2) on DRG neurons is pronociceptive, although the magnitude of the TTX-R Na+ currents depends on the balance of DP1 and DP2 receptor activation. (C) 2011 International Association for the Study of Pain. Published by Elsevier B.V. All rights reserved.”
“Objectives: Formal guidelines recommend that therapeutic hypothermia

be considered after in-hospital cardiac arrest. The rate of therapeutic hypothermia use after in-hospital cardiac arrest and details about its implementation are unknown. We aimed to determine the use of therapeutic hypothermia for adult in-hospital cardiac arrest, whether use has increased over time, and to identify factors associated with its use.\n\nDesign: Multicenter, prospective cohort study.\n\nSetting: A total of 538 hospitals participating in the Get With the Guidelines-Resuscitation database PF-02341066 (2003-2009).\n\nPatients: A total of 67,498 patients who had return of spontaneous circulation after in-hospital cardiac arrest.\n\nInterventions: None.\n\nMeasurements and Main Results: The primary outcome was the initiation of therapeutic hypothermia. We measured the proportion of therapeutic

hypothermia patients who achieved target temperature (32-34 degrees C) and were overcooled. Of 67,498 patients, therapeutic hypothermia was initiated in 1,367 patients (2.0%). The target temperature (32-34 degrees C) was not achieved in 44.3% of therapeutic hypothermia patients within 24 hours and 17.6% were overcooled. The use of therapeutic hypothermia increased from 0.7% in 2003 to 3.3% in 2009 (p < 0.001). We found that younger age (p < 0.001) and occurrence in a non-ICU location (p < 0.001), on a weekday (p = 0.005), and in a teaching hospital (p = 0.001) were associated with an increased likelihood of therapeutic hypothermia being initiated.\n\nConclusions: After in-hospital cardiac arrest, therapeutic hypothermia was used rarely.