Lowest observed effect concentrations (LOEC) and 100% lethal concentrations (LC100) data were collected from the scientific literature. Comparisons were made amon Data on low dissolved oxygen (DO2) tolerance of freshwater fish species of north-western Eurg life stages as well as between native and exotic species. In addition, lethal DO2 concentrations were compared to oxygen concentrations corresponding to maximum tolerable water temperatures of the same species. Fish eggs and embryos were the least tolerant. Juveniles had a significantly NSC 750424 lower mean LOEC than adults,

but there was no difference in mean LC100 between the two groups. The difference in lethal oxygen concentrations between adults and juveniles was largest for three salmonids,

although it remains uncertain if this was a result of smoltification. There were no significant differences between native and exotic species; however, data on exotics are limited. DO2 concentrations converted from maximum tolerable water temperatures were 39 times higher than the measured lethal DO2 concentrations, which may reflect changes in respiration rates (Q(10)) and may also relate to the Selleckchem Compound Library simplicity of the model used. (C) 2013 The Authors Journal of Fish Biology (C) 2013 The Fisheries Society of the British Isles”
“Introduction: PrestoBlue is a new resazurin based reagent to assess cell viability and cytotoxicity. It is claimed to be a fast and highly sensitive assay. Here, we compared PrestoBlue, alamarBlue, and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazoniumbromide (MTT) in assessing cell viability of human corneal epithelial cells (HCEC), and investigated the click here effect of plate color, reading mode, and plate storage on the performance of PrestoBlue assay. Methods: The viability of different numbers of healthy HCEC and the toxicity of various chemicals on HCEC were evaluated

using PrestoBlue (fluorescence), alamarBlue (fluorescence), and MTT (absorbance). The sensitivities of the three assays were compared. In the PrestoBlue assay, three plate colors and two reading modes were used and compared in assessing the toxic effect of sodium dodecyl sulfate (SDS). The PrestoBlue solutions after reaction were stored and measured on day 1, 2, 3, 5, and 7. The fluorescence readings obtained on different days were then compared. Results: Both PrestoBlue and alamarBlue were able to detect 5000 healthy cells after 30 min incubation and 1000 cells after 1 h, 2 h, and 4 h incubation; while MTT was able to detect 5000 cells after 3 h incubation. In the assessment of the toxicity of various chemicals, PrestoBlue and alamarBlue performed similarly. There was no significant difference between the results obtained by these two reagents. All the three plate colors and two reading modes showed similar results in the PrestoBlue assay in assessing the toxicity of SDS. Plate storage up to 7 days did not affect the result of the PrestoBlue assay.