Methods: Unadulterated plasma samples were analyzed directly, following a simple filtration, by the use of a small extraction column, containing a restricted access material, combined with a monolithic analysis column in a column-switching HPLC system.
Results: Up to 4 ml of plasma was analyzed by this method within 4.5-7 min in a fully automated process. Because of the rapid analysis, a large number of samples could be analyzed during a 90-min PET
scan. The extraction column could be used for analysis of up to 500 ml of plasma before replacement was required.
Conclusions: The described method is fast and robust and the large sample check details volumes allow for accurate determination of the radioactive components in plasma even at 90 min after injection of a [C-11]-labeled radiopharmaceutical. (C) 2009 Elsevier Inc. All rights reserved.”
“Purpose: Penile torsion is a congenital malformation that results in a rotational defect of the penile shaft. Various techniques have been described for correcting penile torsion, although there is no consensus on the best repair. We describe our experience using a dorsal dartos flap to correct penile torsion.
Materials and Methods: We retrospectively reviewed the records of all 25 patients who underwent repair of significant penile torsion using a dorsal dartos flap at our
institution between 2004 and 2007. A total of 17 repairs were performed in association with chordee repair, 7 with hypospadias and 1 with bilateral find more inguinal. hernias. Patient age at the time of repair ranged from
6 to 19 months (mean 9). Of the patients 15 had torsion of at least 90 degrees, 8 had torsion of 60 to 90 degrees and 2 had torsion that was not recorded in degrees.
Results: Mean followup was 4 months (range 1.5 to 19). Of the cases 16 demonstrated complete resolution of penile torsion, 7 had residual torsion less than 10 degrees and 2 had documented improvement that was not reported in degrees. No patient has undergone further repair for torsion.
Conclusions: see more Penile torsion is a challenging congenital anomaly. The dorsal dartos flap is familiar to pediatric urologists and can be varied for use in repair of penile torsion. The procedure results in successful repair of the torsion, has few complications and can easily be performed concurrently in the setting of other operative repairs. This approach provides excellent short-term results.”
“Introduction: Cell preparation procedures injurious to Na/I symporters (NIS) could deter their usefulness for reporter gene assays and in vivo cell imaging. in this study, we investigated the effects of cell collection by trypsinization on radioiodide transport and in vivo cell imaging results.
Methods: The influence of trypsinization procedures on (125)I transport was evaluated using Huh-7/NIS hepatoma cells.