Mice were injected subcutaneously with 1 × 105 breast cancer cells in 0.1 ml of PBS. Mice of the control

group (n = 6) were injected with 1 × 106 autologous PBMC, and verum group mice (n = 6) were injected with 1 × 106 autologous CAPRI cells every second day until day 15. PBMC and CAPRI cells were introduced surrounding the injected tumour locations. Mice were observed for 45 days after cancer cell injection. Tumour size was measured for the first time after 21 days. Mice were killed if the maximum tumour diameter was >15 mm unless the tumour killed the mouse before that point. After 45 days, the experiment was completed, and all mice were killed. Pictures were taken with a Konica Minolta Dimage Z3 camera (Konica Minolta Business MG-132 cost Solutions Deutschland GmbH, Langenhagen, Deutschland), and figures were prepared with corel PHOTO-PAINT, version 12.0.0.536.,

and Adobe Illustrator CS5, version 3.0.0.400. NVP-AUY922 manufacturer Patient panel, CAPRI cell dose and treatment schedule.  All steps of the production of autologous activated immune cells including the final therapy (treatment attempts) were controlled by the medical doctor (RW) himself. In Germany, medical doctors are allowed to perform such treatment attempts on their own authority. The preparation of CAPRI cells as well as the treatment was performed at the Institute of Immunology of the Ludwig-Maximilians-Universitaet (LMU), München. The patients’ survival data from the Munich Tumor Center were collected from several hospitals, from gynaecologists and from surgeons, independently from the type of treatment, the type of chemotherapy

or radiation therapy. In essence, the data from the Munich Tumor Center are a summary of individual case reports like those from patients treated with CAPRI cells. Each breast cancer patient (T1-4N0-2, G2-3) with diagnosed metastasis (M1, N = 42) who had received at least 500 × 106 CAPRI cells (although higher cell amounts were recommended and often received) was included in the analysis and compared to breast cancer patients with the same tumour staging (T1-4N0-2M1, G2-3) of the Munich Tumor Center (N = 428). Inclusion for treatment was independent of the type of chemotherapy, radiation and/or other therapies. The recommended Methane monooxygenase treatment schedule included three injections of 60–80 × 106 CAPRI cells per week for 6 months, which was followed by two injections per week for another 6 months. ACT with CAPRI cells has continued for most of the patients once a week for several years. One-third of CAPRI cells were injected i.v., and two-thirds were given i.m. into the forearm in a 1 ml volume of PBS. Statistical analysis.  The slope and y intercept of the regression lines obtained from CML titrations were evaluated using the general linear model (GLM) procedure. The statistical package spss 10.1 (SPSS Inc., Chicago, IL, USA) was used.