Microbial Ecol 1986, 12:65–78.CrossRef 56. Selvam K, Vishnupriya B, Subash Chandra Bose V: Screening and quantification of marine actinomycetes producing industrial enzymes

amylase, cellulase and lipase from South cost of India. Int J Pharma Biol 2011, MAPK inhibitor 2:1481–1487. 57. Jang H-D, Chen K-S: Production and purification of thermostable cellulases from Streptomyces transformant T3–1. World J Microbiol Biotechnol 2003, 19:263–268.CrossRef Competing interests The authors declare that they have no competing interest. Authors’ contribution Research concept and the experiments were performed by BM and LAR, NVV and RK analyzed the data and reviewed the manuscript. All authors approved the final manuscript.”
“Background Pseudomonas aeruginosa is the major pathogen involved in the decline of lung

function in patients with cystic fibrosis (CF) [1–5]. Its presence in the lungs is associated with an increased mortality and morbidity of Selleck VS-4718 CF patients [6]. Early detection of this bacterium from respiratory tract is determinant because it ensures effective patient management [5, 7, 8]. Indeed, after intermittent colonization by different strains, once acquired, chronic P. aeruginosa colonization by mucoid and biofilm-growing isolates is difficult to eradicate [2, 4, 9, 10]. Thus, the earlier the treatment toward P. aeruginosa onset, the higher the chance to efficiently control P. aeruginosa [5, 7, 8]. However, accurate identification of this bacterium in CF sputum by selleck conventional microbiology techniques is known

to be limited. This can be explained by a large phenotypic diversity of P. aeruginosa isolates recovered from CF patients such as loss of pigment production or exopolysaccharide production. Moreover, Singh et al. demonstrated that P. aeruginosa can form biofilms in the airways of CF patients [11]. Biofilms contain bacterial cells that are in a wide range of physiological states. One of the mechanisms 17-DMAG (Alvespimycin) HCl contributing to this physiological heterogeneity includes the adaptation to the local environmental conditions. For instance, bacterial cells from the deep layers of biofilm depleted of oxygen [12] can grow in anaerobic conditions. Therefore, the CF patients isolates obtained from biofilms, i.e. in anaerobic conditions, grow hardly in aerobic conditions on a conventional culture medium [13]. Another limitation of conventional culture is that P. aeruginosa can be easily misidentified with closely related Gram-negative bacilli in CF sputum [14–19]. The use of molecular techniques such as PCR could improve accurate identification of P. aeruginosa [14–19], and consequently, its early detection in CF sputum patients [20–24]. To date, there is no consensus for a universal protocol for the molecular detection of P. aeruginosa. Indeed, its genome is known to be highly polymorphic. Changes that can occur at the genetic level could compromise the reliability of molecular identification techniques.