Mycobacterial HSP65, which has about 50% homology with the human homologue HSP60 [38] serve as the carrier for the diabetogenic peptide P277, may interact with B cells. Recent studies show that HSPs enhance delivery and cross-processing of HSP-linked Ag by B cells [35] and [39]. Although the effects of antigen presentation by various antigen-presenting cells to cloned CD4+ T cells in vitro has not been tested in the present study, the idea has been

established that dendritic cells and macrophages promote antigen-specific Th1 cell differentiation, and B cell presentation of antigen usually induces T cell anergy and tends to promote naïve T cell differentiation toward an anti-inflammatory Th2 phenotype [40], [41], [42] and [43]. Interestingly, T cells from the HSP65-6 × P277 treated mice when incubated with P277 the pattern of cytokine showed an increase in IL-10 and a decrease in IFN-γ (Fig. 4). If activated P277-specific B cells see more serve as APCs and present HSP65-6 × P277 to T cells, it might be promote antigen-specific

www.selleckchem.com/products/sch-900776.html Th2 cell differentiation. Moreover, the capacity of Th2 cells to function as T-helper cells for antibody production is severely hampered in the control mice, which recruited lower levels of P277-specific B cells than HSP65-6 × P277 treated mice (Fig. 1). It is conceivable that the absence of P277-specific B cells to act as antigen-presenting cells may be responsible, in part at least, for the decrease of Th2 cell differentiation in the HSP65 and P277 treated mice. In this study, the mice immunization with the fusion protein HSP65-6 × P277 elicited much higher levels of Th2-type cytokines and lower Th1-type cytokines than the control mice (P < 0.05). A possible explanation for the enhanced Th2-regulated immune response in HSP65-6 × P277 treated mice is that when P277-specific B cells are recruited, the dramatic increase in levels of IL-4 or other Th2-type cytokines. This occurs by the modulation of

the homing of autoreactive cells to inflammatory sites and the stabilization of a protective Th2-mediated environment in the pancreatic islets ( Fig. 2 and Fig. 3). Thus, IL-4 favors the expansion of regulatory CD4+ Th2 cells in vivo that would normally be subject to promote retention of the Th2 phenotype. The respiratory tract is a less acidic Dichloromethane dehalogenase and proteolytic environment and it has been an attractive route of immunization. Nasal administration of autoantigen decrease organ-specific inflammation has been tested experimentally in several models of autoimmunity. For example, nasal administration of HSP65 in mice lacking the receptor for LDL can cause significant decrease in the size of atherosclerotic plaques, and suppress inflammation and atherosclerosis development [16]. Weiner HL et al showed that nasal administration of amyloid A-β peptide limits decreased amyloid plaque deposition in a transgenic animal model of Alzheimer’s disease [44].