On the other hand, if PSII is excited more strongly than PSI, the consequent loss of Φ PSII is reflected by a proportional loss of Φco2. Wavelengths in the range around 480 nm (blue) result in the strongest preferential excitation of PSII and therefore the strongest loss of both Φco2 and Φ PSII (Hogewoning et al. 2012). However, Φ PSII is also an unreliable measure of Φco2 for these blue wavelengths, due

to the absorption by carotenoids and non-photosynthetic pigments (see above). In summary, Φ PSII calculated Quizartinib purchase from chlorophyll a fluorescence measurements is an unsuitable parameter for estimating the wavelength dependence of Φco2. Wavelength-dependent changes in (1) the absorbed light fraction, (2) the light fraction

absorbed by photosynthetic carotenoids, and (3) the light fraction absorbed by non-photosynthetic pigments, directly affect the fraction of GW786034 ic50 photons reaching the photosystems and therefore Φco2. However, at low light intensities, changes in the fraction of photons reaching the photosystems may not affect Φ PSII. Furthermore, (4) some wavelengths preferentially excite PSI, resulting in high Φ PSII values but low Φco2 values. As a consequence, for a reliable measurement of the wavelength dependence of Φco2, gas exchange measurements remain the gold standard. Question 31. Can anthocyanins and flavonols be detected by chlorophyll fluorescence? In vivo non-destructive determination of anthocyanins and flavonols in green parts of plants can be made using the fluorescence excitation ratio method (FER) (Bilger et al. 1997; SHP099 supplier Agati et al. 2011). The FER method is based on the measurement of chlorophyll fluorescence induced by different excitation wavelengths. The extent of absorbance of light by the epidermal polyphenols can be derived on the basis of the ratio of chlorophyll fluorescence emission intensities induced by a standard red beam and a UV–VIS beam (wavelengths strongly absorbed by epidermal polyphenols). Plasmin The role of different anthocyanins and flavonols can be distinguished by choosing appropriate wavelengths based on the specific absorbance spectra of the different anthocyanins

and flavonols. The chlorophyll fluorescence excitation technique was originally developed to assess UV-absorbing compounds in the leaf epidermis (Bilger et al. 1997). Ounis et al. (2001) extended the method developing remote sensing equipment (dual excitation FLIDAR) to study polyphenols not only in leaves but also in canopies of trees. This method has also been used for the determination of the presence of flavonoids, including anthocyanins, in the skins of fruits like grapes (Kolb at al. 2003), apples (Hagen et al. 2006), and olives (Agati et al. 2005). Betemps et al. (2011) showed that in fruits, the anthocyanins and other flavonoids localized in the outer skin layers reduce the chlorophyll fluorescence signal in proportion to the concentration of these polyphenols.