The expression of mRNA for MCP-1 and iNOS was significantly up-regulated at the pretreatment stage compared with healthy controls (P < 0·001 and P < 0·05 respectively), but remained high at the post-treatment stage (P > 0·05) (Fig. 2a). Furthermore, the levels of expression of mRNA for IFN-γ, TNF-α, IL-1β, IL-8, IL-10 and IL-4 were analyzed comparatively in lesions of buy MAPK Inhibitor Library patients treated with

SAG or RFM (Fig. 2b). Three patients treated with SAG and five patients treated with RFM could be followed in this study. To compare the outcome of different treatment regimens in patients with CL, an additional three patients treated with SAG and two treated with RFM (for whom tissue lesions at the pretreatment stage were not available), were also included in the study. There was a significant decrease in the levels of cytokine gene expression in the CL lesions treated with RFM (P < 0·05), whereas no significant decrease was noticed in the levels of IFN-γ, TNF-α and IL-10 (P > 0·05) in lesions treated with SAG. In order to understand the in vivo circulating cytokine profile, serum cytokine levels were analyzed at pretreatment and post-treatment stages in patients with CL and

compared with healthy controls. The level check details of IL-8 was found to be significantly higher in CL samples at the pretreatment stage (1022·4 ± 313·78 pg/ml) compared with the post-treatment stage (10·11 ± 6·97 pg/ml) or the control (10·48 ± 3·9 pg/ml). The level of IL-8 was restored to normal levels after treatment (Fig. 3). The levels of other circulating inflammatory cytokines examined, including

IL-1β, IL-6, IL-10, TNF and IL-12p70, were not detectable in sera. To establish the association between the circulating and localized response of IL-8 and MCP-1, quantitative analysis of IL-8 and MCP-1 was carried out at pretreatment and post-treatment stages in the sera of patients and controls using the more sensitive ELISA method (Fig. 4a). The level of IL-8 determined in the sera (1 : 20 dilution) was found to be significantly higher (P < 0·001) in CL patients (20/20) at the pretreatment stage (89·04 ± 18·8 pg/ml) than in CL patients post-treatment (13·12 ± 5·16 pg/ml) or in controls (5·16 ± 1·45 pg/ml). Similarly, an elevated level of Amine dehydrogenase MCP-1 was observed in all 20 CL patients at the pretreatment stage (39·25 ± 5·29 pg/ml) compared with the controls (21·1 ± 2·6 pg/ml, P < 0·01), but the level of MCP-1 remained high at the post-treatment stage (47·77 ± 3·03 pg/ml, P > 0·05). The circulating nitrite level was analyzed at the pretreatment stage in CL patients (n = 32) and in healthy controls (n = 10), followed by evaluation post-treatment (n = 10) (Fig. 4b). The level of nitrite was significantly higher in CL samples pretreatment (61·37 ± 2·46 μm) than in healthy controls (15·4 ± 0·99 μm, P < 0·001), but the level of nitrite was not significantly down-regulated after treatment (41·1 ± 10·11 μm, P > 0·05).