The following Eurofins methods were used; LMBG L00 00-34, DFG S19

The following Eurofins methods were used; LMBG L00.00-34, DFG S19, GC–ECD learn more for organochlorine pesticides, pyrethroides, PCBs and LMBG L00.00-34, DFG S19, GC–FPD for organophosphorus pesticides. DFG 405, HPLC–FLD for glyphosate and AMPA. Three pooled samples (equal amounts of all individual samples) representing each of the soy categories (GM, conventional and organic) were in addition analysed for the average values of monosaccharides, disaccharides and fibre at the Czech Agriculture and Food Inspection Authority (CAFIA), Za Opravnou

300/6, 150 00 Praha 5, (Czech Republic) and for selected organochloride pesticides OCPs (30 active components including their metabolites) at the National Institute of Nutrition and Seafood Research (NIFES), Bergen, Norway. Organochlorine pesticides (OCPs) were determined by GCMS on a Trace GC 2000 series and Trace DSQ single quadrupole (Thermo Fisher Scientific, Waltham, MA, USA). All samples were collected in Iowa (USA) within a 200 km radius. There were examples of GM-soy and organic soy samples collected within the same town/village (the smallest distance between farms was 5 km). Nine out of ten samples from the conventional soy were sampled in a town or village where most of the GM-soy samples (six out of ten) were also collected. Organic soy and conventional soy samples were not

from the same town/village. The ten samples of conventional Proteases inhibitor soybeans were of four different varieties: Legend 2932 (4 samples), Legend 2375 (3 samples), Asgrow 2869 (2 samples) and Legend 2200. The GM

samples were from 8 to 9 different varieties: Stine 2032 (2 samples), Stine [unnamed], Stine 2538-4, Stine 2602-4, Stine 2062-4, Latham 2158, PB 2217VNRR, PB 2421, Pioneer 92M76. Phosphatidylinositol diacylglycerol-lyase The organic samples consisted of nine different varieties: Pioneer 9305 and ED 4315 (both 2 samples), Legend 2375, Stine 2686, US Soy 20333, Mark 0427, Mark 0431, PB291N and Pioneer 93M52. The conventional and organic varieties overlapped in the use of “Legend 2375” (n = 3 conventional and n = 1 organic sample). There was no overlap in varieties between the GM and either the conventional or organic varieties. Characteristics of the soy samples were analysed with the R-project software with library (vegan) for 35 variables: glycogen, all amino acids, sum of unsaturated, mono- and poly-unsaturated fats, omega3, omega6 and trace elements. Glyphosate and AMPA were first taken out of the primary analyses to look for differences beyond/because of these. In later analyses, concentrations of glyphosate or AMPA and soy variety were included to identify co-variation to other variables. GraphPad Prism 6 (GraphPad Software, San Diego, CA, USA) and Statistica™ 7 (StatSoft Inc., Tulsa, OK, USA) was used to evaluate correlations between nutrient composition and residue levels of glyphosate and AMPA.

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