The percentages of 16S rRNA gene sequence similarity with related organisms were 90.4 % for Thermobacillus xylanilyticus, 91.8% click here for Paenibacillus barengoltzii, 89.4% for Cohnella lupini, 90.1 % for Fontibacillus aquaticus, and 89.0% for Saccharibacillus sacchari. Morphological and physiological

analyses revealed that the strain was motile, rod-shaped, Gram-stain-positive, aerobic and able to form oval endospores in swollen sporangia. Ammonium was required as a nitrogen source while nitrate, nitrite, urea and glutamate were not utilized. Catalase and oxidase activities were weakly positive and positive, respectively. The bacterium grew in the temperature range of 50-65 degrees C and in media with pH 7.5 to 9.0. Optimal growth occurred

at 60 degrees C and pH 8.0-8.6. Growth was inhibited at pH smaller than = 7.0 and NaCI concentrations bigger than = 2.5% (w/v). In chemotaxonomic characterization, MK-7 was identified as the dominant menaquinone. Major fatty acids were iso-C-16:0 and C-16:0. Dominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Phosphatidylcholine was present in a moderate amount. The diamino acid in the cell wall was meso-diaminopimelic acid. The G+C content of the genomic DNA was 49.5 mol% in a nucleic acid study. On the basis of genetic and phenotypic characteristics, strain FAB2(T) (=NBRC 109510(T)=KCTC 33130(T)) showed characteristics AR-13324 suitable for classification as the type strain of a novel species of a new genus in the family Paenibacillaceae, for which the name Ammoniibacillus agariperforans gen. nov., sp. nov. is proposed.”
“Aim. – In France, the Afssaps/HAS 2006 guidelines for insulin-treated type 2 diabetic patients recommend a target glycated haemoglobin level (HbA(1c)) of less than 7%, achieved by optimalizing the insulin dose or increasing the number of daily injections. The present study investigated to what extent these recommendations are followed in clinical

practice by general practitioners (GPs) and diabetologists (DTs).\n\nMethods. Copanlisib – The ADHOC study (observational, transversal) was a survey of 267 GPs and 180 DTs prescribing insulin in France (participation rate: 4.45% and 11.6% of GPs and DTs, respectively). Physicians answered a questionnaire focused on aspects of insulin therapy in type 2 diabetic patients receiving oral antidiabetic drugs (OADs) and insulin for at least six months.\n\nResults. – A total of 1874 patients were included in the study (959 from GPs and 915 from DTs). Insulin was initiated about 10 years after the diagnosis of diabetes, when patients had high HbA(1c) levels (mean value: 9.2%). At the time of the survey, patients had been treated with insulin for 3.4 +/- 3.5 years (mean +/- SD), and the mean HbA(1c) was significantly reduced (P < 0.05) to 7.8% and 7.9% in patients treated by GPs and DTs, respectively.