They did not induce significant cytotoxicity at a concentration of 0.01 g/L after 24 h, and PG[G2]-C(18)-Naph was the least cytotoxic structure after 72 h with values comparable to Cremophor (R) ELP and polysorbate 80. Overall, these amphiphiles possess superior solubilization properties compared to standard excipients used in parenteral formulations with an excellent formulation stability profile and comparable cytotoxicity. (C) 2010
Elsevier B.V. All rights reserved.”
“The resolution of the chicken consensus linkage map has been dramatically improved in this study by genotyping 12,945 single nucleotide polymorphisms (SNPs) on three existing mapping populations in chicken: the Wageningen (WU), BEZ235 inhibitor East Lansing (EL), and Uppsala (UPP) mapping populations. As many as 8599 SNPs could be included, bringing the total number of markers in the current consensus linkage map to 9268. The total length of the sex average map is 3228 cM, considerably smaller than previous estimates using the WU and EL populations, reflecting Geneticin mouse the higher quality of
the new map. The current map consists of 34 linkage groups and covers at least 29 of the 38 autosomes. Sex-specific analysis and comparisons of the maps based on the three individual populations showed prominent heterogeneity in recombination rates between populations, but no significant heterogeneity between sexes. The recombination rates in the F(1) Red Jungle fowl/White Leghorn males and females were significantly lower compared with those in the WU broiler population, consistent with a higher recombination rate in purebred Blebbistatin domestic animals under strong artificial selection. The recombination rate varied considerably among chromosomes
as well as along individual chromosomes. An analysis of the sequence composition at recombination hot and cold spots revealed a strong positive correlation between GC-rich sequences and high recombination rates. The GC-rich cohesin binding sites in particular stood out from other GC-rich sequences with a 3.4-fold higher density at recombination hot spots versus cold spots, suggesting a functional relationship between recombination frequency and cohesin binding.”
“Kompass KS, Deslee G, Moore C, McCurnin D, Pierce RA. Highly conserved transcriptional responses to mechanical ventilation of the lung. Physiol Genomics 42: 384-396, 2010. First published May 11, 2010; doi: 10.1152/physiolgenomics.00117.2009.-Crossspecies analysis of microarray data has shown improved discriminating power between healthy and diseased states. Computational approaches have proven effective in deciphering the complexity of human disease by identifying upstream regulatory elements and the transcription factors that interact with them. Here we used both methods to identify highly conserved transcriptional responses during mechanical ventilation, an important therapeutic treatment that has injurious side effects.