Under the complete coverage of the surface condition, PEG molecules are in direct competition for the adsorption sites on the AuNP

surface [18]. Therefore, the adsorbed linear PEG molecules form typical loops and tail conformations [13, 18]. The value of t is roughly equivalent to the size of the PEG molecule as a free molecule in solution under the condition [13, 18]. Sirolimus mw The root mean square end-to-end length (〈h 2〉1/2) is commonly used to specify the size of a linear polymer molecule. Herein, enlightened by the above facts, we developed a simple and reliable colorimetric method for the MW determination of PEG in aqueous solution using citrate-reduced AuNPs. This method is based on the different stability degrees (SDs) of the AuNPs, which are fully coated

by different MW (〈h 2〉1/2) of PEG, after screening the electrostatic repulsion between nanoparticles. The SDs of the AuNPs are monitored by ultraviolet–visible (UV–vis) spectrophotometry, www.selleckchem.com/products/FK-506-(Tacrolimus).html which exploits the strong sensitivity of the localized surface plasmon resonance spectrum to the aggregation of AuNPs. In this study, the SDs are calculated by the absorbance ratios of the stable to the aggregated AuNPs in solution. The nanoparticles exhibit greater stability upon an increase in the MW (〈h 2〉1/2) of PEG. Of the systems tested, the 〈h 2〉1/2 of PEG molecules was found to exhibit a good linear correlation to the SDs of the AuNPs in a FRAX597 mouse specified range. As a result, we can obtain the 〈h 2〉1/2 of PEG from the SDs of the AuNPs and then estimate the corresponding MW using a mathematical relationship between the 〈h 2〉1/2 and MW of PEG molecule. So far, there is no report on nanomaterial-based methods for the MW determination of polymers. This AuNP-based determination method offers simplicity, Tyrosine-protein kinase BLK convenience, and sensitivity, and can be accomplished in minutes without sophisticated instruments or training overhead. Methods Materials Hydrogen tetrachloroaurate (III) trihydrate (HAuCl4 · 3H2O) and four PEG samples (SPEG 1,450 to 10,000) were purchased from Sigma-Aldrich (St.

Louis, MO, USA). Ten PEG samples (APEG 400 to 20,000) were purchased from Alfa Aesar (Tianjin, China). Trisodium citrate dihydrate (Na3C6H5O7 · 2H2O), sodium azide (NaN3), and sodium chloride (NaCl) were purchased from Sinopharm Group Chemical Reagent Co., Ltd. (Shanghai, China). All chemicals were analytical grade reagents and used without further purification. All water was deionized by reverse osmosis and further purified using a Milli-Q Plus system (Millipore, Billerica, MA, USA) to 18.2 MΩ cm resistivity. All glassware were cleaned using aqua regia solution (HCl/HNO3 = 3:1, v/v) and subsequently rinsed with a copious amount of Milli-Q treated water. AuNP preparation Citrate-reduced AuNPs were prepared according to the modified method [19, 20]. In brief, 99.00 mL of water and 1.00 mL of 1.0% (w/v) HAuCl4 · 3H2O solution were mixed in a flask.