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SP, Sim SH, Jasper LC, Wang D, Liu Y, Ooi EE: Pre- and post-exposure prophylaxis of experimental Burkholderia selleck screening library pseudomallei infection with doxycycline, amoxicillin/clavulanic acid and co-trimoxazole. J Antimicrob Chemother 2008,61(3):674–678.CrossRefPubMed Authors’ contributions BMJ designed and conducted experiments and drafted Selleck LY2874455 the manuscript. GCW contributed to design and conduct next of experiments and drafting manuscript, AGT conducted and provided analysis of the bacterial work, DME conceived the study, participated in its design and coordination and helped to draft the manuscript. All authors read and approved the final manuscript.”
“Background A bacterial cell-to-cell communication mechanism, quorum sensing, is a regulatory process that utilises small, diffusible
signal molecules to modulate specific gene expression in a population density-dependent manner [1, 2]. Diverse gram-negative bacteria can synthesise N-acyl-homoserine lactones (AHLs) as quorum-sensing signal molecules by means of LuxI-type AHL synthases [3]. These quorum-sensing signal molecules share identical homoserine lactone moieties but vary in length or the carbon substitution on the third position on the acyl side chain. As the population density increases, the AHLs bind to LuxR transcriptional regulators; then, the LuxR/AHL complexes regulate the expression of the target genes. The AHL-mediated quorum sensing mechanisms are highly conserved and could regulate infections and virulence factors in several human and plant pathogenic bacteria, such as Chromobacterium violaceum, Burkholderia cepacia, Erwinia carotovora, Brucella melitensis, and Pseudomonas aeruginosa [3–5]. Recently, the AHL-mediated quorum-sensing systems have been viewed as new targets for anti-infective therapies.